Separation of Chlorophyll a, Chlorophyll B, and Beta...

Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance, followed by chlorophyll a. Chlorophyll b is the most polar; therefore, it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is†¦show more content†¦Beta carotene is orange, and its graph created by spectrophotometer shows that it mostly absorbs blue and green light and reflects orange. Figure 1. The absorption spectrum for leaf pigment, wavelength in nm. Discussion During the separation of the pigments by chromatography paper, chlorophyll b traveled the shortest distance, chlorophyll a went above it, and the highest went beta carotene. ThisShow MoreRelatedAp Biologly Photosynthesis Lab Report1336 Words   |  6 Pagesby a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen andRead MoreChromatography1984 Words   |  8 PagesCOLUMN AND THIN LAYER CHROMATOGRAPHY Maria Janine B. Abarientos, Kuia B. Allarde, Aliana Keshia P. Andino Mary Viadelle E. Andrada and Nina Marian Robelea G. Ang Group 1 2C Pharmacy Organic Chemistry Laboratory ABSTRACT In this experiment, the techniques column and thin layer chromatography was used to separate and determine the purity of the colored components of siling labuyo and malunggay leaves. The results obtained relied on differential solubilities and adsorptivities of the components to